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KMID : 0545119910010040246
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Journal of Microbiology and Biotechnology
1991 Volume.1 No. 4 p.246 ~ p.250
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Production and Regeneratin of Lactobacillus bulgaricus Protoplasts
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JUN, HONG KI
PARK, HYUN JEONG/BAIK, HYUNG SUK/SONG, JAE CHUL
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Abstract
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Conditions for the production and regeneration in Lactobacillus bulgaricus protoplasts were investigated. Protoplasts of L. bulgaricus strains were obtained by treatment with mutanolysin and lysozyme together in a protoplast forming buffer containing 0.02 M N-2-hydroxyethylpiperazine-N´-2-ethanesulfonic acid (HEPES) (pH 7.0) and 0.5 M sucrose. High protoplast yield was obtained from cells cultured in the de Man, Rogosa and Sharpe(MRS) medium at the middle to late logarithmic growth phase. Regeneration was efficiently accomplished with a complex medium containing 1% sucrose, 20mM MgCl_2, 5% gelatin, and 0.5% bovine serum albumin. The frequency of regeneration of protoplasts was 10∼20% after 5 days of incubation at 30℃.
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KEYWORD
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